Kinetic properties of epirubicin and morphine glucuronidation in human liver microsomes
| Km | Vmax | Vmax/Km | |
|---|---|---|---|
| μM | pmol/min/mg | μl/min/mg | |
| Epirubicin glucuronide | 149 ± 22 | 99 ± 4 | 0.66 |
| (insect baculosomes) | |||
| Epirubicin glucuronide | 568 ± 130 | 798 ± 87 | 1.40 |
| (human liver microsomes) | |||
| Morphine-3-glucuronide | 1988 ± 225 | 1549 ± 66 | 0.78 |
| (human liver microsomes) | |||
| Morphine-6-glucuronide | 1869 ± 356 | 215 ± 15 | 0.11 |
| (human liver microsomes) |
The kinetic properties of epirubicin glucuronidation in baculosomes specifically expressing UGT2B7 are also indicated. Values are expressed as the mean ± S.E. of two experiments performed in triplicate.