Table 2

Kinetic properties of epirubicin and morphine glucuronidation in human liver microsomes

KmVmaxVmax/Km
μM pmol/min/mg μl/min/mg
Epirubicin glucuronide149  ± 2299  ± 40.66
 (insect baculosomes)
Epirubicin glucuronide568  ± 130798  ± 871.40
 (human liver microsomes)
Morphine-3-glucuronide1988  ± 2251549  ± 660.78
 (human liver microsomes)
Morphine-6-glucuronide1869  ± 356215  ± 150.11
 (human liver microsomes)

The kinetic properties of epirubicin glucuronidation in baculosomes specifically expressing UGT2B7 are also indicated. Values are expressed as the mean ± S.E. of two experiments performed in triplicate.