Table 4

Effect of inhibitors of amine oxidases on the disappearance of tresperimus in rat and human plasma

InhibitorRat PlasmaHuman Plasma
HPLC AnalysisLC/MS AnalysisHPLC AnalysisLC/MS Analysis
% of decrease of tresperimus
None23.9  ± 1.824.0  ± 1.043.4  ± 2.943.00  ± 2.0
Semicarbazide (100 μM)25.1  ± 1.328.0  ± 1.52.9  ± 2.54-a 2.00  ± 6.04-a
Pargyline (1 mM)25  ± 525.7  ± 2.142.1  ± 3.346.00  ± 5.0
Hydralazine (100 μM)23.3  ± 1.321  ± 1.91.1  ± 2.44-a 2.00  ± 3.04-a

The enzyme kinetics were a linear function of substrate concentration and incubation time. Values are means ± S.D. (n = 3) of the percentage of disappearance of tresperimus.

  • 4-a  The high standard deviation seen (relative to the mean result) is probably due to the very low disappearance rate where the contribution of analytical variability becomes predominant.