Kinetic parameters for the N-demethylation of (R)- and (S)-ketamine by microsomes from human B-lymphoblastoid cell lines expressing CYP2B6, CYP2C9, and CYP3A4
| (R)-Ketamine | (S)-Ketamine | |||||
|---|---|---|---|---|---|---|
| Km | Vmax | CLint | Km | Vmax | CLint | |
| μM | pmol/min/pmol CYP | ml/min/pmol CYP | μM | pmol/min/pmol CYP | ml/min/pmol CYP | |
| CYP2B6 | 74.1 ± 34.2 | 33.3 ± 5.0 | 526 ± 264 | 44.4 ± 9.6 | 32.6 ± 3.0 | 750 ± 107 |
| CYP2C9 | 521.4 ± 122.7 | 28.6 ± 1.7 | 57 ± 12 | 756.2 ± 85.8 | 42.1 ± 13.5 | 57 ± 23 |
| CYP3A4 | 711.3 ± 250.2 | 51.6 ± 8.3 | 76 ± 16 | 399.0 ± 48.3 | 42.9 ± 16.4 | 105 ± 30 |
Each value represents the mean ± S.D. of three independent experiments.