Kinetic parameter estimates for flurbiprofen 4′-hydroxylation and piroxicam 5′-hydroxylation in the presence of dapsone
| Dapsone | (S)-Flurbiprofen | Piroxicam | ||
|---|---|---|---|---|
| Km (S.E.) | Vmax(S.E.) | Km (S.E.) | Vmax(S.E.) | |
| μM | μM | pmol/min/pmol P450 | μM | pmol/min/pmol P450 |
| 0 | 28.9 (4.2) | 12.6 (0.5) | 183 (56) | 0.08 (0.01) |
| 2 | 24.8 (4.2) | 15.0 (0.7) | 97.3 (14) | 0.07 (0.003) |
| 5 | 17.6 (2.9) | 16.3 (0.8) | 64.8 (10) | 0.07 (0.002) |
| 10 | 12.6 (1.2) | 17.4 (0.4) | 134 (35) | 0.11 (0.01) |
| 50 | 9.80 (1.1) | 20.7 (0.6) | 53.7 (5.6) | 0.17 (0.004) |
| 100 | 10.0 (1.8) | 20.6 (1.0) | 49.4 (2.0) | 0.20 (0.002) |
Data were fit to the Michaelis-Menten equation (eq. 1).