Table 4

Example calculation of in vitro data extrapolated to in vivo

Male F-344
Vmax = 0.36 nmol/min/0.5 × 106 hepatocytes
1)  0.36 × 2 × 60 = 43.2 nmol/h/106 hepatocytes
2)  Avg. wt. of rats = 279 g
3)  Liver wt. = 4% of body wt.
4)  Avg. liver wt. = 11.19 g
5)  Conversion factor: 128 × 106 hepatocytes/g rodent liver (Kedderis and Held, 1996)
6)  Total hepatocytes in liver = 11.19 g × 128 × 106 = 1431 × 106 hepatocytes
7)  Extrapolate to whole liver = 43.2 nmol/h/106 cells × 1431 × 106 hepatocytes (whole liver) *Total = 61.82 μmol/h