Table 2

Individual enzyme kinetic parameters for (−)-OSU6162 N-despropylation

P450 Enzymes	K_m	V_max	V_max/K_m
	μM	pmol/pmol P450/min
CYP1A1	78.9 ± 8.7	7.23 ± 0.21	0.091
CYP1A2	591.4 ± 146.7	2.58 ± 0.38	0.0043
CYP2C19	194.7 ± 7.8	10.60 ± 0.14	0.054
CYP2D6	4.0 ± 0.3	1.41 ± 0.04	0.351
CYP3A4	439.6 ± 44.7	1.06 ± 0.051	0.0024

Michaelis-Menten parameters (V_max andK_m) and intrinsic clearance (V_max/K_m) were determined from recombinant baculovirus-insect cell microsomes expressing human P450 enzymes.