F and R oligonucleotide primers for the syntheses of competitive reference standard RNA
| Gene | Oligonucleotide Sequence (5′–3′) | Product Size |
|---|---|---|
| crs-MDR1-F | CATTAATACGACTCACTATAGGGA-ATGTCTTCAAATGATTCAAGATCC3-a | Target, 334; crs, 257 |
| T7-Polymerase sequence 1957–1980 | ||
| crs-MDR1-R | ATTCCAAGGGCTAGAAACAATAGTG-ATTATTGCAAATGCTGGTTGCA | |
| 2277–2292 2171–2192 | ||
| crs-CYP3A4-F | CATTAATACGACTCACTATAGGGA-ATATACACACCCTTTGGAAG | Target, 366; crs, 292 |
| T7 Polymerase sequence 1180–1399 | ||
| crs-CYP3A4-R | CATGTACAGAATCCCCGGTTAT-TCTCAGGCACAGATTTCTTG | |
| 1726–1747 1632–1651 | ||
| crs-CYP3A5-F | GTCCTAATACGACTCACTATAGGGA-TCATTCCCAAAGGGTCAAT | Target, 295; crs, 212 |
| T7 Polymerase sequence 1239–1253 | ||
| crs-CYP3A5-R | TTCTGGTTGAAGAAGTCCTTGCG-TCATGAGAGCAAACCTCATG | |
| 1515–1538 1411–1430 |
↵3-a For quantitative PCR, forward primer corresponds to the MDR1 probe sequence in Table 1.