Probe reactions used to characterize enzyme activities, surveyed from 194 drugs approved from US-FDA from 1992 to 1997 period
| Enzymes | Substrate Reactions |
|---|---|
| CYP1A1 | EthoxyresorufinO-deethylation |
| CYP1A2 | PhenacetinO-deethylation, caffeine 3-demethylation, ethoxyresorufinO-deethylation |
| CYP2A6 | Coumarin 7-hydroxylation |
| CYP2B6 | 7-Ethoxy-4-trifluoromethyl coumarinO-dealkylation |
| CYP2C8/9 | Tolbutamide 4′-hydroxylation,S-warfarin 7′-hydroxylation, diclofenac 4′-hydroxylation |
| CYP2C19 | S-Mephenytoin 4′-hydroxylation |
| CYP2D6 | Dextromethorphan O-Demethylation, bufuralol 1′-hydroxylation, debrisoquine 4-hydroxylation, sparteine oxidation |
| CYP2E1 | Chlorzoxazone 6-hydroxylation, debrisoquine, sparteine oxidation, p-nitrophenol |
| CYP3A4/5 | Nifedipine oxidation, testosterone 6β-hydroxylation, erythromycinN-demethylation, cyclosporine oxidation, benzodiazepine hydroxylation (midazolam, triazolam, alprazolam), terfenadine hydroxylation |
| CYP4A | Lauric acid hydroxylation |