Table 1

Primers used to identify CYP3A5 variants

Variant	Location	Name	Primers Seq.(5′-3′)	Position on Reference Sequence (5′)	PCR Fragment (bp)
CYP3A5^2*	Exon 11	^*2(S)	`caatagtactgcatggactcagttgag`	42955	363
		^*2(R)	`cgaccaagttcgtataagacgg`	43317	363
CYP3A5^3*	Intron 3	pri-IN3(S)	`cttcaatttttcactgac ctaatattc`	22724	Primary PCR used pri-IN3(S) and IN3(R) to form 310 bp.
		IN3(S)	`ctttaaagagctcttttgtcctgca`	22868
		IN3(R)	`cacagcatgttgatccccataccta`	23033	Secondary PCR used IN3(S) and IN3(R) to form 166 bp.
CYP3A5^4*	Exon 7	^*4 (S)	`gctgcatgtatagtggaaggac`	30428	318
		^*4 (R)	`tggaattgtaccttttaagtggatg`	30745	318
CYP3A5^5*	Exon 5	^*5 (S)	`catgaagatcaccacaactaatgtg`	28692	252
		^*5 (R)	`cttggaaacggactgtgatcttac`	28943	252
CYP3A5^6*	Exon 7	^*6 (S)	`acaagacccctttgtggagagctttaa`	30570	141
		^*6 (R)	`gacgaaagaactgtatattaagtgtat`	30710	141
CYP3AP1	CYP3AP1 5′-flanking region	3A51F	`ggaagcaacctacatgtccatc`	204	Primary PCR used 3A51F and 3A52R to form 1337 bp
CYP3AP1		3A52R	`atcgccacttgccttcttcaac`	1541	Primary PCR used 3A51F and 3A52R to form 1337 bp
		A⁻⁴⁴G(S)	`caggtgagaggatatttaagaggc`	1205	Secondary PCR used A ⁻⁴⁴G(S) and A⁻⁴⁴G(R) to form 337 bp
		A⁻⁴⁴G(R)	`catcgccacttgccttcttcaac`	1542

The primers of CYP3A5 were designed according to the sequence in GenBank AC005020.5, and the primers of CYP3AP1 were designed according to the sequence in GenBank AF325929.