Nucleotide sequences of primers used in the PCR reactions
| Gene | Forward Primer | Reverse Primer | Amplicon Size |
|---|---|---|---|
| UGT1A1 | 5′ TTTTGTCTGGCTGTTCCCACT | 5′ GAAGGTCATGTGATCTGAATGAGA | 251 |
| UGT1A3 | 5′ ATGTGCTGGGCCACACTCAACT | 5′ TCATTATG(C)AGTAGCTCCACACAA | 130 |
| UGT1A4 | 5′ ACGCTGGGCTACACTCAAGG | 5′ TCATTATGCAGTAGCTCCACACAA | 128 |
| UGT1A6 | 5′ CTTTTCACAGACCCAGCCTTAC | 5′ TATCCACATCTCTCTTGAGGACAG | 289 |
| UGT1A9 | 5′ GAGGAACATTTATTATGCCACCG | 5′ GCAACAACCAAATTGATGTGTG | 118 |
| UGT2B4 | 5′ TCTACTCTTAAATTTGAAGTTTATCCTGT | 5′ TC(A)GCCAGCAGCTCACCA(C)AGGG | 278 |
| UGT2B7 | 5′ AGTTGGAGAATTTCATCATGCAACAGA | 5′ TCAGCCAGCAGCTCAC(C)ACAGGG | 232 |
| UGT2B10 | 5′ TGACATCGTTTTTGCAGATGCTTA | 5′ CAGGTAC(A)TAGGAAGGAGGGAA | 152 |
| UGT2B11 | 5′ CTTCCATTCTTTTTGATCCCAATGATG | 5′ (T)CAGCCAGCAGCTCACCACAGGG | 307 |
| UGT2B15 | 5′ GTGTTGGGAATATTATGACTACAGTAAC | 5′ TCAGCCAG(C)AGCTCACCACAGGG | 141 |
| UGT2B17 | 5′ GTGTTGGGAATATTCTGACTATAATATA | 5′ CAGGTACATAGGAAGGAGGGAA | 242 |
| UGT 1A All | 5′ CGAATCTTGCGAACAACACGAT | 5′ TTCAGGGTCACTCCAGCTCC | 214 |
| UGT 2B All | 5′ AAGTTCTAGGAAGACCCACTAC | 5′ CACCACAACACCATTTTCTCCA | 205 |
| CYP1A2 | 5′ GCCTCGTGAAGAACACTCATG | 5′ CAGGAACCACAGGAACCTCT | 140 |
| 18S RNA | 5′ CGATGCTCTTAGCTGAGTGT | 5′ GGTCCAAGAATTTCACCTCT | 214 |
| TGF-β | 5′ CTGGACACCAACTATTGCTTCA | 5′ AGGGCCAGGACCTTGCTGTA | 188 |
| IL-1β | 5′ ACGATCACTGAACTGCACGCT | 5′ ACACGCAGGACAGGTACAGAT | 209 |
Nucleotides in brackets indicate a mismatch to the gene sequence when downstream primers were shared in PCR.