Table 5

Effects of chlorpyrifos (CPS), permethrin (PMT), and pyridostigmine bromide (PYB) alone and in combination on DEET metabolism by pooled HLM, RLM, MLM, DEET-treated MLM, phenobarbital (PB)-treated MLM, and 3-methylcholanthrene (3-MC)-treated MLM

TreatmentHLMRLMMLM (Control)MLM (DH)MLM (PB)MLM (3-MC)
BALCETBALCETBALCETBALCETBALCETBALCET
nmol product formed/mg protein/min
Control1.1  ± 0.1b 1.5  ± 0.1a 4.3  ± 0.1a 3.1  ± 0.2a 1.3  ± 0.1a 1.7  ± 0.3a 2.0  ± 0.2a 1.9  ± 0.1a 4.8  ± 0.3a 4.3  ± 0.3a 1.3  ± 0.1a 2.0  ± 0.1a
CPS0.3  ± 0.0c 1.4  ± 0.0a 1.1  ± 0.1c 1.6  ± 0.1b c 0.2  ± 0.0b 0.8  ± 0.2b 0.3  ± 0.1b 1.0  ± 0.0b 1.0  ± 0.2b 1.6  ± 0.3b 0.6  ± 0.1b 1.0  ± 0.2b
PMT1.7  ± 0.1a 1.6  ± 0.1a 2.2  ± 0.0b 2.3  ± 0.1a c 0.9  ± 0.2a 1.4  ± 0.2a 1.8  ± 0.4a 2.4  ± 0.5a 2.5  ± 0.2c 3.4  ± 0.3a 0.8  ± 0.1b c 1.7  ± 0.2a
PYB1.8  ± 0.1a 2.1  ± 0.1a 3.6  ± 0.2a 3.0  ± 0.1a 1.2  ± 0.2a 1.8  ± 0.2a 2.2  ± 0.3a 2.8  ± 0.5a 2.9  ± 0.3c 3.0  ± 0.2a 1.2  ± 0.1a c 1.8  ± 0.1a
CPS + PMT0.6  ± 0.0c 1.6  ± 0.3a 1.1  ± 0.2c 1.4  ± 0.0b c 0.3  ± 0.0b 0.8  ± 0.1b 0.3  ± 0.0b 0.9  ± 0.1b 0.4  ± 0.1b 1.1  ± 0.1b 0.5  ± 0.1b 0.7  ± 0.1b
CPS + PYB0.4  ± 0.0c 1.3  ± 0.1a 1.5  ± 0.4b c 2.0  ± 0.3c 0.2  ± 0.0b 0.8  ± 0.1b 0.2  ± 0.1b 0.8  ± 0.2b 0.5  ± 0.0b 1.3  ± 0.1b 0.6  ± 0.0b 0.9  ± 0.0b
PMT + PYB1.6  ± 0.1a 1.6  ± 0.1a 2.5  ± 0.1b 2.4  ± 0.2a c 0.9  ± 0.0a 1.7  ± 0.2a 1.7  ± 0.5a 2.4  ± 0.6a 2.6  ± 0.2c 3.2  ± 0.3a 0.6  ± 0.1b 1.1  ± 0.1b
CPS + PMT + PYB0.6  ± 0.0c 1.4  ± 0.1a 0.9  ± 0.1c 1.3  ± 0.0b c 0.2  ± 0.1b 0.8  ± 0.1b 0.2  ± 0.0b 1.0  ± 0.1b 0.9  ± 0.2b 1.5  ± 0.1b 0.3  ± 0.a 0.9  ± 0.1b

DEET High (DH) 200 mg/kg/day, PB 80 mg/kg/day, 3-MC 20 mg/kg/day. Means in the same column followed by the same letter are not significantly different (P < 0.01). Values are the mean ± S.E.M. (n = 3).