P450 index reactions used
| P450 | Index Reaction | Final Protein Concentration | Incubation Time | References |
|---|---|---|---|---|
| mg/ml | min | |||
| 1A2 | PhenacetinO-deethylation | 2.0 | 25 | Rodrigues et al. (1997) |
| 2A6 | Coumarin 7-hydroxylation | 0.2 | 10 | Shimada et al. (1996) |
| 2B6 | 7-ETC demethylation | 0.2 | 15 | Ekins et al. (1997) |
| 2C9 | Diclofenac 4′-hydroxylation | 0.1 | 20 | Leemann et al. (1993) |
| 2C19 | Omeprazole 5-hydroxylation | 0.4 | 30 | Yamazaki et al. (1997) |
| 2D6 | DextromethorphanO-demethylation | 0.15 | 30 | Rodrigues et al. (1996) |
| 2E1 | Chlorzoxazone 6-hydroxylation | 0.4 | 25 | Lucas et al. (1996) |
| 3A4 | Omeprazole sulfonation | 0.4 | 30 | Yamazaki et al. (1997) |