Table 1

Enzyme kinetic analysis of CYP1A1, CYP1A2, and CYP1B1 inhibition by ginseng extracts

ApparentKiMode of Inhibition
μg/ml
G115
 CYP1A150  ± 21-a Competitive
 CYP1A2906  ± 78Mixed
 CYP1B198  ± 91-a Mixed
NAGE
 CYP1A161  ± 41-a Mixed
 CYP1A220  ± 51-a Mixed
 CYP1B144  ± 31-a Mixed

7-Ethoxyresorufin O-dealkylation assay was performed with human recombinant CYP1A1, CYP1A2, or CYP1B1 at multiple concentrations of the substrate and inhibitor (G115 or NAGE), as indicated in the legends to Figs. 3 and 4. The apparent Ki values and the mode of inhibition were determined as described underMaterials and Methods. Results are expressed as mean ± S.E.M. for three independent experiments.

  • 1-a  Significantly different from the apparent Ki for the inhibition of CYP1A2 by G115, p < 0.05.