Table 1

Primer sequences used for RT-PCR analysis

Primer^1-a	Sequence	GenBank Access Number	Location^1-b	Expected Size
			nt	bp
UGT1A1 sense	`5′-aacaaggagctcatggcctcc`	NM_000463	412–432	646
UGT1A3 sense	`5′-tgttgaacaatatgtctttggtcta`	NM_019093	347–371	698
UGT1A4 sense	`5′-gaaggaatttgatcgcgttac`	AF297093	135686–135706	787
UGT1A5 sense	`5′-ggtggtggtcctcaccctg`	AF297093	129770–129788	701
UGT1A6 sense	`5′-tcctggctgaggtatttgggcc`	NM_001072	560–580	563
UGT1A7 sense	`5′-atgctcgctggacggcaccattg`	U89507	284–306	749
UGT1A8 sense	`5′-ggtcttcgccaggggaatagg`	AF030310	489–518	535
UGT1A9 sense	`5′-ggaggaacatttattatgccaccg`	AF056188	660–683	391
UGT1A10 sense	`5′-cctctttcctatgtccccaatga`	U89508	556–578	477
UGT1A antisense^1-c	`5′-gttcgcaagattcgatggtcg`	AF297093	182646–182627
UGT2B4 sense	`5′-ctcttaaatttgaagtttatcctgt`	NM_021139	249–273	706
UGT2B7 sense	`5′-ttcacaagtacaggaaatcatgtca`	NM_001074	341–365	592
UGT2B4/7 antisense^1-d	`5′-accacaacaccattttctcc`	NM_021139/NM_001074	954–935 (UGT2B4)
			931–912 (UGT2B7)
UGT2B15 sense	`5′-tgggaatattatgactacag`	NM_001076	373–392	515
UGT2B17 sense	`5′-tgtgttgggaatattctgactataa`	NM_001077	398–422	519
UGT2B15/17 antisense^1-e	`5′-aggggtttggctggtttac`	NM_001076/NM_001077	887–869 (UGT2B15)
			915–899 (UGT2B17)

↵1-a All UGT sense primers correspond to exon 1 sequences in each of the respective UGT genes.
↵1-b Primer locations are identical to those indicated in corresponding GenBank accession numbers.
↵1-c The same exon 3-derived antisense primer was used for all RT-PCR amplifications of family 1 UGTs.
↵1-d The same exon 3-derived antisense primer was used for RT-PCR amplifications of UGT2B4 and UGT2B7.
↵1-e The same exon 2-derived antisense primer was used for RT-PCR amplifications of UGT2B15 and UGT2B17.