Table 1

Apparent kinetic parameters for the glucuronidation of PA and DHA by human liver microsomes (HLM2) and membrane fractions of HK293 cells expressing UGT2B7

μM nmol/mg × min μl/mg × min

HLM2 and UGT2B7 (50 μg of protein) were incubated with PA or DHA (25–750 μM) and [14C]UDPGA (4 mM), as described in the text. The apparent Km and Vmaxwere calculated from the results of two sets of duplicate determinations.