Table 2

Concentration-dependent effect of BSA on pHPPH formation in a pooled human liver microsomal preparation

PHTBSAfuVelocity% of Control
μM % pmol/min/mg protein
50.002.00  ± 0.08 (v a)100
50.1250.926.13  ± 0.26 (v b)2-a 307
50.250.796.11  ± 0.30 (v b)2-a 305
50.500.625.84  ± 0.16 (v b)2-a 292
51.000.435.73  ± 0.23 (v b)2-a 287
502.17  ± 0.07 (v a)100
52.000.304.28  ± 0.14 (v b)2-a 2-b 197
1.50.000.82  ± 0.10 (v c)39
502.17  ± 0.07 (v a)100
54.000.202.63  ± 0.12 (v b)2-a 2-b 121
10.000.56  ± 0.01 (v c)26
  • 2-ap < 0.05 compared with va.

  • 2-bp < 0.05 compared with vc.