Table 3

Concentration-dependent effect of BSA on OH-TLB formation in a pooled human liver microsomal preparation

TLBBSAfuVelocity% of Control
μM % (pmol/min/mg protein)
500.069.1  ± 14.4 (v a)100
500.250.4128.4  ± 7.7 (v b)3-a 3-b 185.9
200.042.5  ± 3.7 (v c)61.6
500.500.1661.7  ± 6.4 (v b)3-b 89.4
100.020.3  ± 1.7 (v c)29.3
501.00.0724.0  ± 3.4 (v b)3-a 3-b 34.8
40.08.1  ± 0.4 (v c)11.8
2500223.6  ± 35.1 (v a)100
2502.00.0649.6  ± 1.4 (v b)3-a 3-b 22.2
150.030.8  ± 0.7 (v c)13.8
25040.0214.5  ± 2.0 (v b)6.5
50.010.2  ± 0.7 (v c)4.6
  • 3-ap < 0.05 compared with va.

  • 3-bp < 0.05 compared with vc.