Effect of BSA on pHPPH formation in human liver microsomes from four preparations
| HLM | PHT | BSA | Velocity | % of Control |
|---|---|---|---|---|
| μM | % | (pmol/min/mg protein) | ||
| HHM-0259 (pooled) | 5.0 | 0 | 2.00 ± 0.12 (v a) | 100 |
| 5.0 | 1.0 | 4.96 ± 0.23 (v b)4-a 4-b | 248 | |
| 5.0 | 2.0 | 4.02 ± 0.10 (v b)4-a 4-b | 201 | |
| 2.0 | 0 | 1.16 ± 0.04 (v c) | 57.8 | |
| 1.5 | 0 | 0.80 ± 0.07 (v c) | 40.0 | |
| P1001 (pooled) | 5.0 | 0 | 2.96 ± 0.14 (v a) | 100 |
| 5.0 | 1.0 | 6.22 ± 0.15 (v b)4-a 4-b | 210 | |
| 5.0 | 2.0 | 4.62 ± 0.25 (v b)4-a 4-b | 156 | |
| 2.0 | 0 | 1.78 ± 0.04 (v c) | 60.2 | |
| 1.5 | 0 | 1.18 ± 0.08 (v c) | 40.0 | |
| 0 | ||||
| HG30 (individual) | 5.0 | 0 | 9.00 ± 0.07 (v a) | 100 |
| 5.0 | 1.0 | 11.29 ± 0.37 (v b)4-a | 125 | |
| 5.0 | 2.0 | 8.62 ± 0.14 (v b) | 95.8 | |
| 2.0 | 0 | 5.58 ± 0.08 (v c) | 62.0 | |
| 1.5 | 0 | 4.02 ± 0.15 (v c) | 44.7 | |
| HG66 (individual) | 5.0 | 0 | 1.93 ± 0.07 (v a) | 100 |
| 5.0 | 1.0 | 3.43 ± 0.08 (v b)4-a 4-b | 177 | |
| 5.0 | 2.0 | 2.64 ± 0.08 (v b)4-a 4-b | 137 | |
| 2.0 | 0 | 1.20 ± 0.07 (v c) | 62.1 | |
| 1.5 | 0 | 0.82 ± 0.04 (v c) | 42.5 |