Catalytic activity of human and animal liver microsomes with fluorometric substrates4-a
| Liver Microsomes Source | Substrate | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| AMMC | MeAMFC | CMC | CEC | MFC | BFC | DBF | BQ | BzRes | |
| Human (pooled, mixed gender) | 3.12 | 5.74 | 917 | 542 | 1744 | 205 | 289 | 3080 | 14 |
| Male SD rat | 313 | 276 | 844 | 491 | 2497 | 302 | 121 | 5369 | 23 |
| Female SD rat | 240 | 156 | 709 | 431 | 1405 | 325 | 50 | 1102 | 6.74 |
| Female DA rat | 16 | —4-c | — | — | 1638 | — | — | — | — |
| Substrate concentration (μM) | 1.5 | 25 | 40 | 40 | 50 | 50 | 2 | 40 | 50 |
| Incubation time (min) | 30 (human) | 45 (human) | 5 | 5 | 5 | 30 | 5 | 5 | 30 |
| 5 (rat) | 10 (rat) | ||||||||
| Protein concentration (mg/ml) | 0.54-b | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
↵4-a Values, expressed as pmol product per minute per milligram of protein, represent the mean of duplicate or with DA rat only triplicate determinations. Ranges or S.D. were within 10% of the mean in all cases.
↵4-b Protein concentration for DA rat was 0.125 mg/ml.
↵4-c Dashes indicate experiment not done.