Fluorometric substrates and their metabolite excitation and emission wavelengths
| Substrate | Substrate Abbreviation | Metabolite | EX | EM |
|---|---|---|---|---|
| nm | ||||
| 3-[2-(N,N-Diethyl-N-methylamino)ethyl]-7-methoxy-4-methylcoumarin | AMMC | (3-[2-(N,N-diethyl-N-methylammonium)ethyl]-7-hydroxy-4-methylcoumarin)1-a | 390 | 460 |
| 3-[2-(N,N-Diethyl-N-methylammonium)ethyl]-7-methoxy-4-trifluoromethylcoumarin | MeAMFC | 3-[2-(N,N-Diethyl-N-methylamino)ethyl]-7-hydroxy-4-trifluoromethylcoumarin | 410 | 538 |
| 7-Methoxy-3-cyanocoumarin | CMC | 7-Hydroxy-3-cyanocoumarin | 410 | 460 |
| 7-Ethoxy-3-cyanocoumarin | CEC | 7-Hydroxy-3-cyanocoumarin | 410 | 460 |
| 7-Methoxy-4-trifluoromethylcoumarin | MFC | 7-Hydroxy-4-trifluoromethylcoumarin | 410 | 538 |
| 7-Benzyloxy-4-trifluoromethylcoumarin | BFC | 7-Hydroxy-4-trifluoromethylcoumarin | 410 | 538 |
| Dibenzylfluorescein | DBF | Fluorescein | 485 | 538 |
| 7-Benzyloxyquinoline | BQ | 7-Hydroxyquinoline | 410 | 538 |
| Resorufin benzyl ether | BzRes | Resorufin | 530 | 590 |
↵1-a 3-[2-(N,N-Diethyl-N-methylamino)ethyl]-7-hydroxy-4-methylcoumarin was used as an external standard for quantification of (3-[2-(N,N-diethyl-N-methylammonium)ethyl]-7-methoxy-4-methylcoumarin).