Table 1

Fluorometric substrates and their metabolite excitation and emission wavelengths

SubstrateSubstrate AbbreviationMetaboliteEXEM
nm
3-[2-(N,N-Diethyl-N-methylamino)ethyl]-7-methoxy-4-methylcoumarinAMMC(3-[2-(N,N-diethyl-N-methylammonium)ethyl]-7-hydroxy-4-methylcoumarin)1-a 390460
3-[2-(N,N-Diethyl-N-methylammonium)ethyl]-7-methoxy-4-trifluoromethylcoumarinMeAMFC3-[2-(N,N-Diethyl-N-methylamino)ethyl]-7-hydroxy-4-trifluoromethylcoumarin410538
7-Methoxy-3-cyanocoumarinCMC7-Hydroxy-3-cyanocoumarin410460
7-Ethoxy-3-cyanocoumarinCEC7-Hydroxy-3-cyanocoumarin410460
7-Methoxy-4-trifluoromethylcoumarinMFC7-Hydroxy-4-trifluoromethylcoumarin410538
7-Benzyloxy-4-trifluoromethylcoumarinBFC7-Hydroxy-4-trifluoromethylcoumarin410538
DibenzylfluoresceinDBFFluorescein485538
7-BenzyloxyquinolineBQ7-Hydroxyquinoline410538
Resorufin benzyl etherBzResResorufin530590
  • 1-a  3-[2-(N,N-Diethyl-N-methylamino)ethyl]-7-hydroxy-4-methylcoumarin was used as an external standard for quantification of (3-[2-(N,N-diethyl-N-methylammonium)ethyl]-7-methoxy-4-methylcoumarin).