Table 1

Michaelis-Menton constant (Km, carrier permeability (Pc) and passive permeability (Pm) for the absorption (AP-BL) and efflux (BL-AP) of CPT-11 across Caco-2, MDCK II/WT, MDCK II/Pgp, and MDCK II/cMOAT cells

Caco-2MDCK II/WTMDCK II/PgpMDCK II/cMOAT
AP to BL
K m(μM)N.D.N.D.N.D.N.D.
P c (cm/s × 105)N.D.N.D.N.D.N.D.
P m (cm/s × 106)0.13  ± 0.020.8  ± 0.20.4  ± 0.10.9  ± 0.2
BL to AP
K m (μM)116.1  ± 15.981.3  ± 14.645.5  ± 7.590.8  ± 6.7
P c (cm/s × 106)13.2  ± 0.76.0  ± 0.422.5  ± 3.511.5  ± 1.8
P m (cm/s × 106)0.17  ± 0.04N.D.N.D.N.D.
  • N.D., Not determinable.