Km | Vmax | Vmax/Km | |
---|---|---|---|
μM | min−1 | min−1 μM−1 | |
CYP2C19 O-Demethylase | |||
2C19 + M → Mono-OH-M | 0.41 ± 0.12 | 39 ± 2 | 95 |
2C19 + Mono-OH-M → Bis-OH-M | 0.23 ± 0.10 | 8.3 ± 0.6 | 35 |
2C19 + Catechol-M → Tris-OH-M | 0.26 ± 0.10 | 14 ± 1 | 54 |
CYP3A4Ortho-Hydroxylase | |||
3A4 + Mono-OH-M → Catechol-M | 12 ± 3 | 15 ± 1 | 1.3 |
3A4 + Bis-OH-M → Tris-OH-M | 25 ± 7 | 4.4 ± 0.6 | 0.17 |
Values represent mean ± S.D. Incubations were conducted for 2 to 15 min. (conditions described under Materials and Methods).