Table 1

Apparent kinetic parameters for the N-glucuronides of S(−)-cotinine and S(−)-nicotine in pooled human liver microsomes

SubstrateKmVmaxVmax/Km
mM pmol/min/mg μl/min/mg
S(−)-Cotinine5.43 ± 0.32695.6 ± 18.90.13 ± 0.06
S(−)-Nicotine1-a 0.11 ± 0.017132 ± 4.51.2 ± 0.27
  • 1-a Data previously reported for the same pooled human liver microsomes (Ghosheh et al., 2001).