Gene-specific oligonucleotide PCR primers
Gene | Forward Primer | Reverse Primer | Base Paira | Reference |
|---|---|---|---|---|
| CYP3A1 | 5′-GGA AAT TCG ATG TGG AGT GC-3′ | 5′-AGG TTT GCC TTT CTC TTG CC-3′ | 239-567 | Hoen et al. (2000) |
| CYP3A2 | 5′-AGT AGT GAC GAT TCC AAC ATA T-3′ | 5′-TCA GAG GTA TCT GTG TTT CCT-3′ | 1247-1497 | Yokogawa et al. (2002) |
| CYP3A9 | 5′-GGA CGA TTC TTG CTT ACA GG-3′ | 5′-ATG CTG GTG GGC TTG CCT TC-3′ | 422-794 | Hoen et al. (2000) |
| CYP3A18 | 5′-TCC TGT CTC CAA CCT TCA CC-3′ | 5′-CAC TCG GTT CTT CTG GTT TG-3′ | 454-866 | Hoen et al. (2000) |
| mdr1a | 5′-GAT GGA ATT GAT AAT GTG GAC A-3′ | 5′-AAG GAT CAG GAA CAA TAA A-3′ | 1997-2347 | Vos et al. (1998) |
| mdr1b | 5′-GAA ATA ATG CTT ATG AAT CCC AAA G-3′ | 5′-GGT TTC ATG GTC GTC GTC TCT TGA-3′ | 1942-2267 | Vos et al. (1998) |
| GAPDH | 5′-CCA TCA CCA TCT TCC AGG AG-3′ | 5′-CCT GCT TCA CCA CCT TCT TG-3′ | 1061-1636 | Vos et al. (1998) |
↵ a Area amplified refers to sequences deposited at GenBank with accession numbers as follows: L24207 (CYP3A1), U09742 (CYP3A2), U46118 (CYP3A9), D38381 (CYP3A18), AF257746 (mdr1a), AY082609 (mdr1b), and AF106860 (GAPDH)