TABLE 2

Detection of P450 mRNAs and proteins in mouse small intestine

P450 mRNA expression was detected by RNA-PCR. P450 protein expression was detected by immunoblotting analysis.


P450 Form

mRNA (Constitutive)

Protein


Constitutive Induced
1A1 + +(weak) +++a(BNF)
1A2 -
1B1 + +(weak) + (BNF)
2A4/5 -
2A12 -
2B9b +
2B10 + +(CYP2B) ++ (PB)
2B19 +
2B20 +
2C29 +
2C37 - +(CYP2C) ++ (PB)
2C38 +
2C39 -
2C40 +
2E1 + + ND
2F2 -
3A11 +
3A13 + +(CYP3A) ++ (DEX)
3A16 +
3A25 +
3A44
+


  • +, positive detection of DNA fragments of predicted size or P450-immunoreactive band; -, product of predicted size or immunoreactive band not detected. ND, not determined.

  • a +, low induction from very low constitutive expression; ++, 3- to 5-fold induction; +++, > 10-fold induction

  • b CYP2B9 can only be detected in PB-treated mice.