TABLE 1

Acetaminophen-protein adducts

Mass	Fraction	Protein
kDa
Proteins identified by isolation and sequence analysis^a
44	Microsomes	Glutamine synthetase
50	Mitochondria	Glutamate dehydrogenase
54	Mitochondria	Aldehyde dehydrogenase
55—58	Cytosol	Selenium (acetaminophen) binding protein
74—75	Nucleus	Lamin-A
100	Cytosol	N-10 Formyl THF dehydrogenase
Proteins identified by mass spectrometry^b
22	Cytosol, mitochondria	Glutathione peroxidase
28	Mitochondria	House keeping protein
29	Cytosol, microsomes	Thioether S-methyltransferase
16	Cytosol	Aryl sulfotransferase
32	Not known	Pyrophosphatase
29	Cytoskeleton	Tropomyosin 5
22	Cytosol, mitochondria	GSH peroxidase
52	Cytosol	Selenium binding protein
52	Cytosol	Acetaminophen binding protein
28	Cytosol	Proteasome subunit C8
45	Cytosol	Methionine adenosyl transferase
46	Ribosomes	Protein synthesis initiation factor 4A
56	Mitochondria	Aldehyde dehydrogenase
54	Cytosol	Aldehyde dehydrogenase
59	Mitochondria	ATP synthetase α-subunit
29	Cytosol	Carbonic anhydrase III
35	Peroxisomes	Urate oxidase
36	Mitochondria, peroxisome	2,4-Dienoyl-CoA reductase
22	Cytosol (macrophages)	Osteoblast-specific factor 3
23	Cytosol, mitochondria	Glutathione transferase π
40	Cytosol	Sorbitol dehydrogenase precursor
32	Cytosol	Glycine N-methyltransferase
32	Cytosol	3-Hydroxyanthraniliate 3,4-dioxygenase

THF, tetrahydrofuran.
↵ a Cohen et al. (1997)
↵ b Qiu et al. (1998)