Table 1

Effect of PCP on 7-EFC O-deethylation activity, P450 CO-reduced spectrum, and absolute spectrum of P450 2B6

Sample	% Activity Remaining		% P450 Remaining		% Absolute Spectrum
Sample	0 min	40 min	0 min	40 min	0 min	40 min
−PCP, −NADPH	100	92 ± 11	100	82 ± 3	100	86 ± 6
⁺PCP, −NADPH	108 ± 10	91 ± 10	88 ± 9	84 ± 5	87 ± 8	80 ± 6
⁺PCP,⁺NADPH	109 ± 17	39 ± 5	84 ± 4	78 ± 7	82 ± 14	88 ± 11

Reconstituted P450 2B6 was incubated in the presence or absence of PCP and NAPDH as described under Materials and Methods. Each value represents the mean and S.D. of three separate experiments.