Enzyme System | KI | kinactivation | t1/2 |
---|---|---|---|
μM | min−1 | min | |
Expressed P450 2B6 | 5.1 | 0.09 | 7.7 |
Human liver microsomes | 1.8 | 0.09 | 7.7 |
P450 2B6 activity was measured as DCMB hydroxylation using either recombinant enzyme or human liver microsome as described underMaterials and Methods. Apparent KI andkinactivation values represent single determinations resulting from linear regression analysis of the double reciprocal plots.