Table 2

Primers used for site-directed mutagenesis

PrimerSequenceaLocationb
MP-C64T 5′-ACTCCTGGTTCAGTGCCACCCTAACAC-3′ 51–77  (27)
MP-G516T 5′-CACCTTCCTCTTCCATTCCATTACCGCCAAC-3′ 501–531  (31)
MP-C777A 5′-CCTGGACCCCAGAGCCCCCAAGGAC-3′ 765–789  (25)
MP-A785G 5′-CCAGCGCCCCCAGGGACCTCATCGA-3′ 773–797  (25)
MP-C1459T 5′-CAACATACCAGATCTGCTTCCTGCCCCGC-3′ 1445–1473  (29)

All primers were 5′-phosphorylated.

  • a Bold letters indicate mutations produced by PCR-based site-directed mutagenesis.

  • b Values in parentheses indicate number of bases (-mer).