Concentration-dependent effects of endogenous steroids on CBZ 10,11-epoxidation by expressed CYP3A4 and CYP3A7
| Added Steroids | CYP3A4 | CYP3A7 |
|---|---|---|
| μM | nmol/nmol P450/min (%) | nmol/nmol P450/min (%) |
| none | ||
| 0 | 3.275 (100.0) | 0.062 (100.0) |
| AND | ||
| 25 | 6.229 (190.2) | 0.104 (167.6) |
| 50 | 8.944 (273.1) | 0.127 (205.7) |
| 100 | 9.769 (298.3) | 0.134 (216.5) |
| 200 | 9.320 (284.6) | 0.159 (256.6) |
| EST | ||
| 25 | 3.249 (99.2) | 0.032 (51.8) |
| 50 | 3.017 (92.1) | 0.023 (37.5) |
| 100 | 2.857 (87.2) | 0.023 (36.7) |
| 200 | 3.085 (94.2) | 0.021 (33.8) |
| DHEA-S | ||
| 25 | 3.190 (97.4) | 0.155 (251.4) |
| 50 | 2.774 (84.7) | 0.184 (296.9) |
| 100 | 3.163 (108.5) | 0.194 (330.9) |
| 200 | 3.414 (104.3) | 0.215 (348.1) |
The concentration of substrate was 100 μM.