Table 3

Kinetic parameters of AND 6β-hydroxylation by expressed CYP3A4

Km1 (apparent)Km2(apparent)Vmax2Vmax2/Km2
μM nmol/nmol P450/min
AND only3.5100.3117.491.17139
Plus CBZ2.097.881.370.83200

Substrate concentrations were 5 to 500 μM. The reaction was performed in the absence or presence of 100 μM CBZ.