Table 4

Kinetic parameters of DHEA-S 16α-hydroxylation by expressed CYP3A7

Km(apparent)VmaxVmax/Km
μM nmol/nmol P450/min
DHEA-S only17.06.600.3882
Plus CBZ21.56.050.2814

Substrate concentrations used were 10 to 500 μM. The reaction was performed in the absence or presence of 100 μM CBZ.