Table 2

Effect of cryopreservation on viability of rat hepatocytes after 2 h of incubation2-a

Freshly Isolated CellsMethod 12-bMethod 22-b
70.3  ± 0.357.5  ± 7.02-c 70.5  ± 3.2
  • 2-a  Viability was assessed by trypan blue exclusion after 2 h of incubation in Krebs-Henseleit buffer as described under Materials and Method. Data are expressed in percentage of viable cells ± S.E.M.

  • 2-b  Isolated rat hepatocytes were cryopreserved following two different protocols: Method 1 and Method 2 as described under Materials and Methods.

  • 2-c  Viability of cells from Method 1 is significantly different from that of fresh cells (p < 0.05) for n = 4.