Table 1

Enzyme kinetic parameters for fluvoxamine inhibition of (S)-mephenytoin metabolism and free fractions of fluvoxamine in microsomal incubations from six human livers

Human Liver	K_m	V_max	K_i,total	Free Fraction^1-a	K_i,ub
	μM	nmol/min/mg protein	nM	f _u,mic	nM
HL105	37	0.54	323	0.27	87
HL114	21	0.16	201	0.34	68
HL134	28	0.25	185	0.37	68
HL135	33	0.068	241	0.32	77
HL152	43	0.057	256	0.31	79
HL157	39	0.048	201	0.38	76
Mean	34	0.19	235	0.33	76
S.D.	7.9	0.19	51	0.04	7.1

Protein concentration is 0.5 mg/ml.

↵1-a Fluvoxamine spiked concentration was 0.3 μM.