Table 2

Nonspecific binding of bufuralol fluoxetine, ezlopitant, imipramine, and quinidine to Sf9 cell microsomes and phospholipid

Unbound Fraction
Microsomal protein concentration (mg/ml)
Phospholipid concentration (mg/ml)

The extent of binding for bufuralol, quinidine, and imipramine was minimal in the absence of additional microsomal protein or phospholipid. The unbound fractions for fluoxetine and ezlopitant at 8.8 μg/ml were 0.68 and 0.05, respectively.