Table 2

Nonspecific binding of bufuralol fluoxetine, ezlopitant, imipramine, and quinidine to Sf9 cell microsomes and phospholipid

	Unbound Fraction
	Bufuralol	Fluoxetine	Ezlopitant	Imipramine	Quinidine
Microsomal protein concentration (mg/ml)
0.2	0.91	0.16	0.041	0.47	0.80
0.5	0.58	0.051	0.016	0.34	0.63
2.0	0.44	0.025	0.0029	0.23	0.46
Phospholipid concentration (mg/ml)
0.2	0.78	0.14	0.023	0.43	0.75
0.5	0.65	0.099	0.0039	0.38	0.46
2.0	0.30	0.061	0.00033	0.15	0.31

The extent of binding for bufuralol, quinidine, and imipramine was minimal in the absence of additional microsomal protein or phospholipid. The unbound fractions for fluoxetine and ezlopitant at 8.8 μg/ml were 0.68 and 0.05, respectively.