Table 4

Kinetic parameters of tolperisone hydroxylation in baculovirus-infected insect cell expressed human P450s

KmVmaxVmax/Km
μM pmol/min/pmol P450 μl/min/pmol P450
CYP1A2160.6  ± 7.123.6  ± 5.780.15  ± 0.04
CYP2C1924.4  ± 8.633.7  ± 9.161.47  ± 0.54
CYP2D61.8  ± 0.299.3  ± 2.465.16  ± 1.03

Parameters were calculated using nonlinear regression-based methods. Data are represented as mean of three-independent experiments ± S.D