Table 2

Kinetic analysis of exogenous compounds glucuronidation by UGT2B17

SubstrateVmaxKmGlucuronidation Efficiency (Vmax/Km)
pmol/min · mg protein μM (l · min−1mg−1 · 10−8)
Alizarin125.319.5642.6
Eugenol10217.9569.8
Galangin5842.1137.8
Scopoletin102.578.9129.5

Values of apparent Km andVmax were determined as described underMaterials and Methods. Results represent the mean of two independent experiments each performed in triplicate.