Methods to identify pathways involved in the oxidative biotransformation of a compound
In Vitro System | Condition | Tests |
|---|---|---|
| HLM | ± NADPH | P450, FMO vs. other oxidases |
| HLM, hepatocytes | ± 1-Aminobenzotriazole | Broad specificity P450 inactivator |
| HLM | 45°C pretreatment | Inactivates FMO |
| S-9 | ± Pargyline | Broad MAO inactivator |
|
S-9, cytosol
|
± Menadione, allopurinol
|
Mo-CO inhibitors
|