Substrate concentrations, incubation times, contents of P450, and internal standards used in the present study
Reaction | Substrate | Incubation Time | P450 | Internal Standard |
|---|---|---|---|---|
| μM | min | pmol | μg | |
| POD | Phenacetin (10) | 15 | 5 | Caffeine (0.25) |
| DFH | Diclofenac (25) | 20 | 5 | Zaltoprofene (0.1) |
| BLH | Bufuralol (10) | 15 | 0.5 | None |
| PNPH | p-Nitrophenol (200) | 60 | 5 | Phenacetin (0.125) |
| MD4H | Midazolam (25) | 20 | 10 | Nitrazepam (0.125) |
|
T7H/T16H
|
Testosterone (25)
|
30
|
10
|
None
|