Real-Time PCR Primers and Probes | Position | |
---|---|---|
CYP3A4 | ||
Forward primer | 5′ CATTCCTCATCCCAATTCTTGAAGT 3′ | exon 8 |
Reverse primer | 5′ CCACTCGGTGCTTTTGTGTATCT 3′ | exon 8/exon 9 |
TaqMan probe | 5′ CGAGGCGACTTTCTTTCATCCTTTTTACAGATTTTC 3′ | exon 8 |
CYP3A5 | ||
Forward primer | 5′ GCTCGCAGCCCAGTCAATA 3′ | exon 10 |
Reverse primer | 5′ AGGTGGTGCCTTATTGGGC 3′ | exon 10/exon 11 |
TaqMan probe | 5′ TGAAACCACCAGCAGTGTTCTTTCCTTCAC 3′ | exon 10 |
CYP3A43 | ||
Forward primer | 5′ AATACGAACATTGCTATCTCCAGCT 3′ | exon 5 |
Reverse primer | 5′ GCTTCTCACCAACATATCTCCACAT 3′ | exon 6 |
TaqMan probe | 5′ TTCACCAGTGTAAAATTCAAGGAAATGGTCCC 3′ | exon 5/exon 6 |
hPXR | ||
Forward primer | 5′ ACCTTTGACACTACCTTCTCCCAT 3′ | exon 4 |
Reverse primer | 5′ CGCAGCCACTGCTAAGCA 3′ | exon 5 |
TaqMan probe | 5′ TCAAGAATTTCCGGCTGCCAGGG 3′ | exon 4/exon 5 |
huPO | ||
Forward primer | 5′ CCATTCTATCATCAACGGGTACAA 3′ | 791-810a |
Reverse primer | 5′ AGCAAGTGGGAAGGTGTAATCC 3′ | 842-863a |
TaqMan probe | 5′ TCTCCACAGACAAGGCCAGGACTCGT 3′ | 811-840a |
↵ a Position refers to sequence in GenBank Accession No. M17885