TABLE 1

Sequences and positions of primers and probes used for real-time quantitative PCR.




Real-Time PCR Primers and Probes

Position
CYP3A4
Forward primer 5′ CATTCCTCATCCCAATTCTTGAAGT 3′ exon 8
Reverse primer 5′ CCACTCGGTGCTTTTGTGTATCT 3′ exon 8/exon 9
TaqMan probe 5′ CGAGGCGACTTTCTTTCATCCTTTTTACAGATTTTC 3′ exon 8
CYP3A5
Forward primer 5′ GCTCGCAGCCCAGTCAATA 3′ exon 10
Reverse primer 5′ AGGTGGTGCCTTATTGGGC 3′ exon 10/exon 11
TaqMan probe 5′ TGAAACCACCAGCAGTGTTCTTTCCTTCAC 3′ exon 10
CYP3A43
Forward primer 5′ AATACGAACATTGCTATCTCCAGCT 3′ exon 5
Reverse primer 5′ GCTTCTCACCAACATATCTCCACAT 3′ exon 6
TaqMan probe 5′ TTCACCAGTGTAAAATTCAAGGAAATGGTCCC 3′ exon 5/exon 6
hPXR
Forward primer 5′ ACCTTTGACACTACCTTCTCCCAT 3′ exon 4
Reverse primer 5′ CGCAGCCACTGCTAAGCA 3′ exon 5
TaqMan probe 5′ TCAAGAATTTCCGGCTGCCAGGG 3′ exon 4/exon 5
huPO
Forward primer 5′ CCATTCTATCATCAACGGGTACAA 3′ 791-810a
Reverse primer 5′ AGCAAGTGGGAAGGTGTAATCC 3′ 842-863a
TaqMan probe
5′ TCTCCACAGACAAGGCCAGGACTCGT 3′
811-840a
  • a Position refers to sequence in GenBank Accession No. M17885