TABLE 2

Variability in IC50 values observed in intra- and interday experiments for inhibitors used as controls in each 96-well plate

Values are IC50 ± S.D.



1A2 (Furafylline)

2C9 (Sulfaphenazole)

2C19 (Ticlopidine)

2D6 (Quinidine)

3A4 (Ketoconazole)

Intraday
Interday
Intraday
Interday
Intraday
Interday
Intraday
Interday
Intraday
Interday
Conventional 1.5±0.23 1.8±0.66 2.1±0.41 2.4±0.29 0.35±0.05 0.39±0.04 0.005±0.001 0.005±0.0006 0.040±0.01 0.047±0.0078
Fluorescent
1.1±0.11
1.3±0.69
0.32±0.03
0.27±0.06
0.16±0.03
0.17±0.015
0.016±0.001
0.017±0.0025
0.018±0.003
0.019±0.0027