TABLE 2

List of PCR primers

Gene
Accession Number
Sequence 5' to 3'
Primer Annealing Temperature
Sizea
Forward Primer
Reverse Primer
°C base pairs
CYP1A1 NM_000499 ttc gtc ccc ttc acc atc ctg aat tcc acc cgt tgc 56 302
CYP1A2 NM_000761 tcg taa acc agt ggc agg t ggt cag gtc gac ttt cac g 60 254
CYP2B6 b NM_000767 atg ggg cac tga aaa aga ctg a aga ggc ggg gac act gaa tga c 62 283
CYP2C9 NM_000771 ctg gat gaa ggt ggc aat tt aga tgg ata atg ccc cag ag 59 308
CYP2D6 b NM_000106 cta aggg aac gac act cat cac ctc acc agg aaa gca aag aca c 60 289
CYP2E1 NM_000773 gac tgt ggc cga cct gtt act acg act gtg ccc ttg g 58 297
CYP3A4 NM_000776 att cag caa gaa gaa caa gga ca tgg tgt tct cag gca cag at 64 314
CYP3A7 NM_000765 ggt tca gta aaa aga aca agg aca a tgg ggc aca gct ttc tta aa 66 306
EPHX1 NM_000120 cca cct gga cca ata cgg tcc cca cga acc atg tag 56 301
UGT1A6 c NM_001072 tcc tgg ctg agt att tgg gcc gtt cgc aag att cga tgg tcg 65 562
GSTM1 XM_002155 agc caa agt act tgg agg aac ctc ctc act ccc acc tcc t 58 319
SULT1A1 NM_001055 cat gga cca cag cat ctc c cga act cct ggg ctc aaa t 60 308
NAT1 NM_000662 gga tgc cat gga ctt agg c ggc tga tcc ttc cca gaa at 56 297
NQO1 NM_000903 agg cag tgc ttt cca tca tcc tgc ctg gaa gtt tag gt 56 284
Luciferase AF434931 tga caa gga tgg atg gct ac ctt gac tgg cga cgt aat cc 60 281
GAPD d NM_002046 acc aca gtc cat gcc atc ac tcc acc acc ctg ttg ctg ta 60 452
HMBS NM_000190 ttc gct gca tcg ctg aaa tgc ccc aca aac cag tta at 60 368
EF1A1 NM_001402 cag caa aaa tga ccc acc aa tca aag cga ccc aaa ggt 60 291
HPRT NM_000194 cct gct gga tta cat caa agc act g cct gaa gta ttc att ata gtc aag 58 369
ATP5O
NM_001697
acc caa gga gtc gtt tct gc
tta gac aat ctc ccg cat agc
58
282

  • EPHX1, microsomal epoxide hydrolase; GST, glutathione S-transferase; UGT, UDP-glucuronosyltransferase.

  • ↵ a Expected size of the PCR product.

  • ↵ b Primer sequences were taken over from Rodriguez-Antona et al. (2000).

  • ↵ c Primer sequences were taken over from Strassburg et al. (1997).

  • ↵ d Primer sequences were taken over from Ko et al. (1999).