TABLE 2
Protein concentrations used in all described assays.
Isoform
Radiometric/Fluorometric
MS
Final Assay Conditions 1/5 Protein MS
pmol/ml
3A4
25
25
5
2C9
70
25
5
2C19
40
12.5
2.5
2D6
20
25
5
1A2
15
75
15
Total
NA
162.5
32.5