Oligonucleotide primers used for amplification and cloning of UGTs 1A7, 1A8, and 1A10
No. | Name | Sequencea |
|---|---|---|
| 1 | 1A9HindF | TTTGAAGCTTACATTAATGCTTCTGGAGAACA |
| 2 | 1A7-5F | TCTTTTGAGGGCAGGTTCTATCT |
| 3 | 1A8-5F | CTCTTATAGTACTCTTATGAGATAC |
| 4 | 1A10-5F | TCACTTGCAGCGTGCTCTCCCT |
| 5 | 1A7-3R | ATTATGATTCTGTAAGAAATCTTTTAAT |
| 6 | 1A8-3R | CAATTCAGTAAGGAATCTTTTTTTAAT |
| 7 | 1A10-3R | GAAAGAAATTTGAAATGCAACAAACGA |
| 8 | 1A78-BamF | TCTGGATCCAGAATCCCAGCTGCTGGCTC |
| 9 | 1A10-BglF | TCTAGATCTAGAATCCCAGCTGCTGGCTC |
|
10
|
1A7810-HindR
|
TGTAAGCTTCAAATTCCATAGGCAMTGGCTTTCCCTGA
|
↵ a All the sequences are in the 5′ to 3′ direction. New restriction sites are underlined