TABLE 1

Sulfation rates of catechols by human recombinant sulfotransferases a

Compound
1A1
1A2
1A3
1B1
1C2
1E1
nmol/mg/min
Endogenous Compounds
    1 Dopamine 4 -b 8.5 - - -
    2 6-Hydroxydopamine 3 - 0.8 1.1 - -
    3 5-Hydroxydopamine - - 1.4 - - -
    4 Adrenaline 0.2 - 4.1 - - -
    5 Noradrenaline 0.8 - 6.7 - - -
    6 Salsilinol 11 0.2 11.6 0.3 - -
    8 l-Dopa 4.9 0.5 2.9 0.5 - -
10 3,4-Dihydroxyphenylacetic acid 0.9 0.1 0.2 - - -
11 3,4-Dihydroxymandelic acid 0.6 0.4 0.3 0.17 - -
12 3,4-Dihydroxyphenylglycol 8.9 - 1.7 0.7 - -
13 2-Hydroxyestradiol 7.5 2.8 13.5 6.1 - 1.8
14 4-Hydroxyestradiol 3 1.5 1 0.1 - 3.7
15 4-Hydroxyestrone 5 3.5 0.4 0.3 - 3.6
Drugs and Pharmacological Tools
16 Apomorphine 14.2 2.2 9.8 0.7 - 2.6
17 Dihydrexidine 2.2 0.2 1.3 0.3 - 0.3
18 Dobutamine 21.3 1.8 8 1.4 - 0.3
19 SKF38393 10 0.1 12.8 - - -
20 Carbidopa 15.6 0.4 4.5 1.6 - -
21 Tyrphostin A23 1.1 0.3 2.2 1.4 0.1 0.5
22 Ethyl-3,4-dihydroxybenzylcyanoacetate - 0.5 1 0.8 - 0.6
23 2-(1-Thienyl)ethyl-3,4-dihydroxybenzylcyano acetate - - 0.8 0.3 - 0.4
24 Entacapone 0.52 - 0.6 - - -
25 Tolcapone - - 0.3 - - -
26 3,5-Dinitrocatechol - - - - - -
Natural Compounds
27 Gallic acid - - - - - -
28 Methyl gallate - - - - - -
29 Catechin 4.1 1.4 1.5 1.3 - -
30 Epicatechin 2.6 0.2 2.3 0.7 - -
32 Epicatechin gallate - - - - - -
33 Epigallocatechin gallate - - - - - -
34 Rosmarinic acid - - 0.1 0.2 - -
35 Chlorogenic acid 0.2 - - - - -
Various Chemicals
36 Catechol 8.8 6.9 7.1 5.4 - 0.2
37 Pyrogallol 1.2 0.2 0.2 - - -
38 3-Methoxycatechol 11.1 14.5 12.8 11.8 - 2.3
39 2,3-Dihydroxybenzoic acid - - 0.3 - - -
40 2,3-Dihydroxybenzaldehyde 4.3 4.6 3 - - -
41 3-Fluorocatechol 8.9 9.2 8.1 11 - 0.7
42 3-Chlorocatechol 2.3 6.2 10 10.6 - 1.2
43 4-Methylcatechol 14.5 9.7 10.1 13.2 - 0.5
44 4-Isopropylcatechol 17.2 16.8 22.4 24.5 - 1.4
45 4-tert-Butylcatechol 19.6 17.3 19.3 27.2 - 5.7
46 4-Chlorocatechol 6.3 7.1 12.2 11.7 - 0.3
47 4-Nitrocatechol 2 2.9 2.9 2.5 0.2 0.6
48 3,4-Dihydroxyacetophenone 7.4 1.6 6.7 10.8 - 0.8
49 3,4-Dihydroxybenzoic acid ethyl ester 13.1 8.2 14.2 14.9 0.1 3.97
50 4-tert-Butyl-5-methoxycatechol 12.2 11.5 6.4 12.6 - 7
51 3-Methoxy-5-bromocatechol 0.1 0.4 1.3 2.8 - 1.3
52 Tetracholorocatechol - 0.1 3.4 2.3 0.1 1.5
53 2,3-Dihydroxynaphthalene
15.5
11
16.8
20.1
0.2
5.8

  • SKF38393, 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine

  • ↵ a The enzyme activities were determined as described under Materials and Methods

  • ↵ b -, no sulfation could be determined (the limit of determination was 0.1 ng/mg/min)