TABLE 1

Purification of the enzyme involved in 5′-DFCR formation from capecitabine in human liver cytosol


Step

Volume

Total Protein

Total Activitya

Specific Activitya

Purification

Recovered Activity
ml mg pmol/min pmol/min/mg protein fold %
Cytosol 110.0 4459.5 10,156.0 2.3 1.0 100.0
40 to 60% (NH4)2 SO4 35.0 1423.7 6170.9 4.3 1.9 60.8
Sephacryl S-300 46.6 135.9 6138.0 45.2 19.8 60.4
Mono P 9.2 6.9 1267.1 183.4 80.5 12.5
Superdex 200
21.2
1.2
465.7
399.4
175.4
4.6
  • a 5′-DFCR formation activity.