TABLE 1

Kinetic analysis of Mrp2-mediated E23G transport in the presence of E217G or E23SO417G

Nonlinear regression results from data in Fig. 4, C and D are presented. Data were fitted as discussed in the text, with Vmax shared for all data sets. Bold type indicates 95% CI different from control.




Control

E217G (5 μM)

E217G (25 μM)

E217G (125 μM)

E23SO417G (5 μM)
Best-Fit Values
Vmax (pmol·mg-1·min-1) 326 326 326 326 326
Hill coefficient 0.880 0.884 0.91 0.981 0.932
S50 (μM) 55.7 88.8 89.9 82.4 99.5
95% Confidence Intervals
Vmax (pmol·mg-1·min-1) 286-366 286-366 286-366 286-366 286-366
Hill coefficient 0.848-0.912 0.867-0.902 0.882-0.938 0.968-0.994 0.918-0.945
S50 (μM)
44.2-70.1
79.3-99.5
75.8-107
76.7-88.6
91.5-108