Steroid | RT | Selected Ion (Ions Quantified)a | Characteristic Ions | a b | b b | Linearityc | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
[M·]+ | -CH3 | -CH3O (-MOX) | -(CH3)3SiO (-TMS) | |||||||||
min | m/z | m/z | ||||||||||
ADIONE | 8.91 | 344,329 | 344 | 329 | 0.225 | 0.000461 | 0.912 | |||||
Etio | 9.60 | 270,360 | 360 | 270 | IS | IS | IS | |||||
7α-OH-DHEA | 9.68 | 387,356 | 356 | 387 | 0.722 | 0.0225 | 0.991 | |||||
DHEA | 10.40 | 268,358 | 358 | 268 | 0.172 | 0.00965 | 0.985 | |||||
16α-OH-DHEA | 10.54 | 446,356 | 446 | 356 | 0.112 | 0.0059 | 0.943 | |||||
7β-OH-DHEA | 10.79 | 387,477 | 477 | 387 | 0.468 | 0.00984 | 0.976 | |||||
7-oxo-DHEA | 13.92 | 432,401 | 432 |
| 401 |
| 0.0124 | 0.0000345 | 0.935 |
RT, retention time; Etio, etiocholanolone (3α-hydroxy-5β-androstan-17-one); IS, internal standard.
↵ a Ions used for quantitative analysis are underlined
↵ b a = slope = relative mass response = mean peak area ratio of steroid × mass of IS/mass of steroid; b = y-intercept
↵ c Linearity is represented by the linear correlation coefficients of the calibration curves for each standard