TABLE 3

Sulfoxidation activity toward phorate with P450 and FMO isoforms Values are the mean ± S.E.M. (n = 3).




Km

Vmax

CLint

%TNR
μM nmol/nmol μl/nmol
isoform/min isoform/min
1A2 43.7 ± 7.8 86.0 ± 5.6 1970 20.2
3A4 50.1 ± 7.9 49.0 ± 2.9 980 24.2
2B6 32.1 ± 4.9 70.8 ± 3.6 2210 10.4
2C9*1 23.6 ± 2.7 35.2 ± 1.2 1500 32.9
2C18 8.9 ± 1.8 20.8 ± 0.9 2330 1.3
2C19 22.9 ± 3.9 57.2 ± 3.0 2490 10.8
FMO1
48.8 ± 8.7
26.2 ± 1.8
540
0.1