TABLE 6

Sulfoxidation activity toward methiocarb with P450 and FMO isoforms Values are the mean ± S.E.M. (n = 3).




Km

Vmax

CLint

%TNR
μM nmol/nmol μl/mol
isoform/min Isoform/min
1A2 16.6 ± 3.2 26.6 ± 1.7 1600 12.1
3A4 17.8 ± 3.1 27.9 ± 2.1 1570 28.4
2B6 11.0 ± 2.5 28.0 ± 1.9 2550 8.8
2C9*1 4.1 ± 0.6 10.2 ± 0.4 2450 39.4
2C19 5.5 ± 1.2 15.2 ± 1.1 2760 8.8
2D6*1 11.2 ± 2.2 14.0 ± 1.2 1250 2.1
FMO1
30.1 ± 5.7
86.2 ± 6.7
2860
0.5
  • N.D., not determined.